Human NEBL (Nebulette) ELISA Kit

A colorimetric Sandwich ELISA from Assay Genie for the in vitro quantitative determination of human Nebulette (NEBL) in serum, plasma, tissue homogenates, and other biological fluids. The kit uses a double-antibody sandwich detection format and is reactive with human samples.

Reported sensitivity is 0.188ng/ml with a working range of 0.313-20ng/ml. Spike-and-recovery testing yielded average recoveries of 99% for serum (range 89-105%), 92% for EDTA plasma (range 86-99%), and 96% for UFH plasma (range 89-104%). Linearity was demonstrated across 1:2, 1:4, and 1:8 serial dilutions in serum, EDTA plasma, and UFH plasma. Intra-assay precision is reported as CV<8% and inter-assay precision as CV<10%. The kit is intended for research use only, with components stored at 4°C for 6 months.

Supplied components include a dismountable 8×12-strip ELISA microplate, lyophilized standard, sample/standard dilution buffer, biotin-labeled antibody, antibody dilution buffer, HRP-streptavidin conjugate (SABC), SABC dilution buffer, TMB substrate, stop solution, 25X wash buffer, and plate sealer.