Human NDUFB8 ELISA Kit (HUFI07530)

NDUFB8 sits within the peripheral arm of mitochondrial Complex I, anchoring the iron-sulfur cluster assembly that funnels electrons from NADH toward ubiquinone, and its expression is widely tracked as a proxy for mitochondrial content in skeletal muscle, cardiac tissue, and neural cultures. Loss-of-function variants in NDUFB8 have been linked to Leigh syndrome and progressive neurodegeneration, while reduced NDUFB8 immunoreactivity is reported in Parkinson's substantia nigra and in type 2 diabetic islets, making quantitative readouts useful for studies of OXPHOS deficiency. Immunoassay quantification complements Western blot and Blue Native PAGE workflows by delivering absolute concentration values rather than band densitometry, especially when working with limited biopsy or biobank material. The kit is supplied through Assay Genie's small-batch manufacturing program with a frozen lyophilized standard curve that yields seven non-zero calibrators plus a blank for accurate interpolation. Reconstituted standards should be aliquoted and stored at -20°C to limit freeze-thaw cycles, and the kit's 100 uL sample volume per well matches standard automated liquid handling protocols used in high-throughput mitochondrial research pipelines.