Human NDUFB10 ELISA Kit (HUFI07542)

NDUFB10 (NADH:ubiquinone oxidoreductase subunit B10) is a nuclear-encoded accessory subunit of the mitochondrial respiratory chain Complex I. It localises to the inner mitochondrial membrane and contributes to electron transfer from NADH to ubiquinone, supporting oxidative phosphorylation and cellular ATP production.

This Assay Genie immunoassay is intended for research use only and is applicable to investigations of mitochondrial bioenergetics, metabolic disease mechanisms, and oxidative stress responses in human-derived specimens. Capture and detection antibodies are provided as a matched pair in a microplate format, and the procedure is compatible with standard plate-reader instrumentation used in biomedical research laboratories.

Upon receipt, refrigerate the kit between uses; allow all reagents to equilibrate before each run, and aliquot reconstituted standards to minimise repeated freeze-thaw exposure. Protect light-sensitive components from prolonged illumination, and refer to the enclosed protocol for recommended dilutions, incubation times, and assay validation data.