Human ND2 ELISA Kit (HUFI08707)

This Assay Genie immunoassay enables quantitative measurement of human NADH dehydrogenase subunit 2 (ND2), a mitochondrial DNA-encoded core subunit of respiratory Complex I. The 96 Assays kit is configured for colorimetric readout on standard microplate readers, supporting mid-throughput studies of oxidative phosphorylation, mitochondrial bioenergetics, and respiratory chain integrity. Researchers use this platform to investigate mitochondrial disorders including LHON-spectrum optic neuropathy, MELAS-related pathophysiology, and neurodegeneration-linked Complex I deficiency, often profiling ND2 abundance alongside other mtDNA-encoded subunits to map assembly and stability of the NADH-ubiquinone oxidoreductase holoenzyme. Each kit includes the assay reagents for the complete protocol, which is intended for research use only and is not validated for clinical diagnostic procedures. Reagents require refrigerated storage to preserve antibody binding activity across the shelf life of the product, with assay steps carried out at room temperature incubation. The format is suited to academic laboratories, mitochondrial disease research groups, and biotech translational programs working with human-derived specimens. The colorimetric endpoint is captured in the visible spectrum without the need for specialized fluorescence or luminescence instrumentation, simplifying integration into existing plate-based workflows in core facilities and individual labs alike.