Human NCOA3 ELISA Kit (HUFI07549)

Nuclear Receptor Coactivator 3 (NCOA3), also called SRC-3, AIB1, and p/CIP, is a 160 kDa member of the p160 coactivator family whose abundance in research samples can be quantified with the Assay Genie Human NCOA3 ELISA Kit.

NCOA3 interacts with ligand-activated nuclear receptors, including estrogen receptor alpha and progesterone receptor, recruiting chromatin-remodeling machinery to target gene promoters. Because NCOA3 is amplified in a subset of hormone-responsive breast tumors, tracking its protein abundance is widely applied in breast cancer research, endocrinology investigations, and studies of endocrine-therapy resistance.

Each package supplies reagents sufficient for 96 Assays in a microplate layout, intended for research use only and not for diagnostic procedures. Components include pre-coated plate wells, calibrated standards, detection antibodies, and substrate reagents needed to complete the immunoassay workflow. Upon arrival, store the kit refrigerated and protect from light to maintain reagent performance through the stated shelf life.

Researchers commonly pair this assay with western blot validation, cell culture stimulation experiments, and immunohistochemistry on tissue sections to confirm findings across protein, cellular, and histological readouts. Investigations of estrogen-receptor-positive tumor models, prostate cancer signaling pathways, and metabolic regulation through nuclear receptor activity routinely use this NCOA3 measurement to quantify responses to hormonal, pharmacological, or genetic perturbations in vitro and in vivo.