Human NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1) ELISA Kit

The NDUFS1 protein is the 75 kDa iron-sulfur subunit of mitochondrial respiratory chain Complex I (NADH:ubiquinone oxidoreductase), and quantifying its abundance supports research into oxidative phosphorylation, mitochondrial bioenergetics, and complex I deficiency disorders such as Leigh syndrome. Mutations disrupting this subunit impair electron transfer at the NADH-binding module and are linked to lactic acidosis, hypertrophic cardiomyopathy, and neurodegeneration phenotypes studied in patient-derived fibroblasts and animal tissue models.

This Assay Genie reagent is supplied as a microplate configured for 96 assays and accommodates cell lysate and tissue homogenate inputs, with reactivity validated in human, mouse, and rat samples. Investigators working on Parkinson's disease models, metabolic syndrome, and exercise-physiology interventions use this ELISA to monitor NDUFS1 protein levels across treatment cohorts.

Handle the kit following the enclosed insert; protect reagents from light, store at the temperature indicated in the manual, and aliquot working samples before freezing to limit freeze-thaw degradation of mitochondrial antigens. Each well requires 100 µL of sample, and the 96-assay format provides sufficient wells for standard curve replicates and multiple biological replicates per plate.