Human NAD-dependent deacetylase sirtuin-6 (SIRT6) ELISA Kit

Assay Genie's Human NAD-dependent deacetylase sirtuin-6 (SIRT6) ELISA Kit is a sandwich-format immunoassay intended for the quantitative measurement of SIRT6 in human samples. It uses a colorimetric ELISA detection method and is supplied for research use only, supporting investigations in the epigenetics research area.

The kit reports a detection range of 78-5000pg/mL and a sensitivity of 20pg/mL. It has been validated for use with serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, with specificity for natural and recombinant human NAD-dependent deacetylase sirtuin-6 (UniProt: Q8N6T7). The target analyte, also known by the synonyms SIR2-like protein 6 and SIR2L6, is an NAD-dependent protein deacetylase located predominantly in the nucleoplasm and associated with telomeric heterochromatin regions.

SIRT6 functions as an NAD-dependent protein deacetylase with activity towards 'Lys-9' and 'Lys-56' of histone H3, modulating acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. It has been implicated in DNA repair, glucose metabolism, and aging, with dysregulation linked to cancer, cardiovascular disorders, and neurodegenerative conditions.