Human Na-K-Cl Cotransporter 2 (NKCC2) ELISA Kit

The thick ascending limb of the loop of Henle depends on NKCC2 (Na-K-Cl Cotransporter 2), a renal-specific ion transporter encoded by the SLC12A1 gene. Located in the apical membrane of tubular epithelial cells, NKCC2 mediates sodium, potassium, and chloride reabsorption and serves as the molecular target of loop diuretics such as furosemide and bumetanide. Loss-of-function mutations in SLC12A1 cause Bartter syndrome type I, a hereditary tubulopathy characterized by renal salt wasting, polyuria, hypokalemia, and metabolic alkalosis. Researchers in nephrology, hypertension, and renal pharmacology use this Assay Genie ELISA Kit to quantify NKCC2 protein in tissue homogenates and cell lysates from human samples. The kit supports 96 assays in a microplate format, enabling measurement of renal tissue specimens, primary tubular cell cultures, and experimental models of loop of Henle function. Detection relies on absorbance measurement, and the assay is intended for research use only, not for diagnostic procedures. Applications include studies of tubular sodium handling, salt-sensitive hypertension mechanisms, and pharmacological profiling of compounds acting on the SLC12A1 transporter.