Human N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit

This NT-proBNP immunoassay kit from Assay Genie targets the N-terminal pro-B-type natriuretic peptide, a 76-amino acid prohormone fragment cleaved from proBNP during secretion by cardiac ventricular myocytes. Circulating concentrations of NT-proBNP rise sharply in response to increased ventricular wall stress, making the analyte a cornerstone biomarker in cardiovascular research on congestive heart failure, left ventricular hypertrophy, myocardial ischemia, and acute coronary syndromes.

The assay accepts a diverse array of human biospecimens including blood fractions (serum or plasma), tissue homogenates, cell lysates, and cell culture supernatants, supporting translational workflows from in vitro cell-based experiments through clinical sample analysis. Each kit contains reagents supplied in a microplate format sufficient for 96 individual determinations, with all components intended for research use only.

For optimal reagent stability, kit components should be refrigerated upon arrival and protected from light. Researchers frequently pair NT-proBNP measurement with complementary cardiac markers such as BNP, cardiac Troponin I, and cardiac Troponin T to construct multi-analyte panels characterizing myocardial stress, fibrosis, and post-infarct remodeling. Assay Genie provides detailed standard curve protocols, recommended dilution schemes, and matrix-effect guidance for sample types where endogenous peptide concentrations are elevated.

This product supports immunoassay workflows in academic cardiology laboratories, biomarker validation studies, pharmaceutical screening programs investigating drug-induced cardiotoxicity, and translational research programs developing novel heart failure therapeutics.