Human N-Acylsphingosine Amidohydrolase 1 (ASAH1) ELISA Kit

Acid ceramidase (ASAH1) is a lysosomal hydrolase that converts ceramide into sphingosine and a free fatty acid, sitting at a key branch point in sphingolipid metabolism that influences apoptosis, inflammation, and membrane signaling. Measuring ASAH1 protein abundance in human specimens supports research into Farber lipogranulomatosis, cystic fibrosis airway biology, and tumor models where ceramide turnover is altered.

Assay Genie supplies this ELISA kit as a complete package with a pre-coated microplate, biotinylated detection reagent, recombinant standards, and substrate solutions sized for one full plate run. The protocol follows a conventional quantitative immunoassay workflow with absorbance readout on a standard plate reader; the supplied calibrators are used to build a standard curve against which unknown samples are interpolated.

Validated for use with human tissue homogenates and cell lysates, the kit is intended for research use only. Unopened components are stored refrigerated until the expiration date on the label, while working dilutions should be prepared fresh and used within the same session to preserve activity.