Human N-Acetyltransferase 1 (NAT1) ELISA Kit

Researchers studying human phase II xenobiotic metabolism can quantify N-Acetyltransferase 1 protein expression directly from tissue homogenate samples using this 96-assay ELISA Kit from Assay Genie. NAT1 is a cytosolic enzyme that transfers acetyl groups from acetyl-CoA onto aromatic amines, heterocyclic amines, and hydrazine derivatives, and its polymorphic expression has been associated with variable drug acetylation capacity and altered susceptibility to arylamine-induced carcinogenesis across different tissues.

The kit supports standard microplate-based immunoassay workflows, with each plate providing 96 assays in a format suitable for running a full standard curve alongside experimental unknowns in duplicate. End-point plate reader quantification yields absorbance values proportional to NAT1 protein concentration in each well, allowing direct comparison across treated versus untreated control samples, patient-derived specimens, and time-course experiments.

Applications include toxicology studies examining how environmental and dietary arylamines are metabolized, oncology research evaluating NAT1 expression patterns in bladder, colorectal, breast, and lung tissues, and pharmacokinetic profiling of amine-containing therapeutics such as isoniazid and sulfamethoxazole. Each kit is supplied with the capture antibody, detection reagent, standards, and buffers required to complete the full plate.