Human N-Acetylneuraminic Acid Synthase (NANS) ELISA Kit

The Human N-Acetylneuraminic Acid Synthase (NANS) ELISA Kit from Assay Genie enables quantitative measurement of NANS protein in human samples using an absorbance-based assay. NANS catalyzes the condensation of phosphoenolpyruvate with N-acetylmannosamine to produce N-acetylneuraminic acid, the biosynthetic precursor of sialic acids that decorate cell-surface glycoproteins and glycolipids. Researchers investigating sialylation pathways, neurodevelopmental disorders linked to NANS mutations, and tumor metabolic reprogramming commonly apply this kit to tissue homogenates and matched experimental samples. The format uses a human-specific capture antibody pre-immobilized on a microplate, with biotinylated detection antibody and streptavidin-HRP conjugate producing signal proportional to analyte concentration. Each pack supplies calibrated standards, wash buffer, and substrate sufficient for 96 individual reactions, and reagents should be aliquoted and stored at the temperatures indicated in the supplied manual. Reconstituted working solutions should be protected from light and the kit is intended for research use only in laboratory settings.