Human N-Acetyl Beta-D-Glucosaminidase (NAGase) ELISA Kit

This Assay Genie sandwich ELISA quantifies urinary N-acetyl-β-D-glucosaminidase (NAGase) activity, a lysosomal hydrolase released from proximal renal tubular cells upon injury. The kit supports nephrotoxicity screening, diabetes-related kidney disease studies, and drug safety pharmacology, where elevated urinary NAGase serves as an early, non-invasive indicator of tubular damage before changes in serum creatinine become apparent.

The plate format provides a detection range of 0.156 to 10 U/L and uses approximately 50 µL of urine supernatant per well, enabling direct measurement without extensive pre-processing. Antibody pairs are calibrated for human NAGase with no significant cross-reactivity with rodent orthologs, supporting human-specific sample analysis in translational research.

Reconstituted reagents should be stored at -20 °C and protected from light, while unused strips return to the sealed pouch with desiccant. To preserve standard curve integrity, researchers commonly aliquot working solutions and limit freeze-thaw cycles. Each kit is supplied with a pre-coated microplate, lyophilised standards, detection antibody, streptavidin-HRP, and wash buffer for straightforward laboratory workflow integration.