Human Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1) ELISA Kit

The MYL1 gene on human chromosome 2q34 (NCBI gene 4632, UniProt P05976) generates two essential light chain isoforms, MLC1F and MLC3F, via alternative splicing; both assemble into the lever-arm neck region of the myosin head and are expressed predominantly in type II (fast-twitch) skeletal myofibers. Bi-allelic MYL1 loss-of-function variants have been linked to severe congenital myopathy with type II fibre hypotrophy, and circulating MYL1 release has been examined as an indicator of sarcolemmal injury in rhabdomyolysis, exhaustive exercise, and inflammatory myopathies, which is the experimental context in which this Assay Genie immunoassay is most often deployed.

To support rigorous quantitative work, the kit reports intra-assay CV below 10% and inter-assay CV below 12%, an assay runtime of roughly 4.5 hours, and a 12-month shelf life from the date of manufacture when stored refrigerated. Researchers typically aliquot reagents to limit freeze-thaw cycles, and the human-specific capture antibody shows no significant cross-reactivity with closely related light chain analogues. This RUO kit is supplied as a complete pack containing a pre-coated microplate, biotinylated detection antibody, standards, and substrate buffers, ready for readout on a standard laboratory plate reader at the wavelength indicated in the manual.