Human Myosin-9 (MYH9) ELISA Kit

A sandwich ELISA from Assay Genie for the quantitative measurement of human Myosin-9 in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The kit uses a double-antibody sandwich format with a biotin-conjugated detection antibody, HRP-conjugated avidin and a TMB substrate, with the resulting optical density read at 450 nm. The product is intended for research use only.

The reported detection range is 15.6–1000 pg/mL, with a sensitivity of 10 pg/mL, an intra-assay CV of 4.9% and an inter-assay CV of 8.1%. Spike-and-recovery in serum averages 83% (range 80–89%) and in plasma 85% (range 80–91%). Specificity covers natural and recombinant human Myosin-9, corresponding to UniProt entry P35579 — a cellular myosin implicated in cytokinesis, cell shape, and specialized functions including secretion and capping. The listed research area is Signal Transduction, and the target is reported in the cytoplasm, cytoskeleton and cell cortex.