Human Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) ELISA Kit

Apoptosis and oncology research often requires precise quantitation of MCL1, an anti-apoptotic BCL-2 family member anchored in the outer mitochondrial membrane that restrains BAX/BAK-mediated permeabilization and sustains cell survival under stress. Because MCL1 turns over rapidly and is regulated both transcriptionally and post-translationally, accurate measurement in cell-derived and tissue-derived samples is a routine need in studies of cancer cell survival, chemoresistance, and BCL-2 family inhibitor response. This Assay Genie colorimetric immunoassay supports quantitative measurement of human MCL1 (Myeloid Cell Leukemia Sequence 1, BCL2 related) and is supplied as a complete 96-assay kit.

Validated for human reactivity with sample types including tissue homogenates and cell lysates, the kit provides capture and detection antibodies, recombinant standards, and the buffers required to generate a calibration curve on a standard microplate reader. The colorimetric endpoint allows absorbance-based readout in routine plate workflows, and the 96-assay layout supports standard curves alongside replicate wells for each biological sample.

Typical applications include profiling MCL1 expression in cancer cell lines and primary tumor specimens, investigating resistance mechanisms to chemotherapeutics and BCL-2 family inhibitors, monitoring MCL1 changes following pro-apoptotic stimuli, and supporting studies in hematological malignancies where MCL1 is recognized as a survival dependency. The kit is intended for in vitro research use only, and reagents should be stored and handled according to the manufacturer's product insert to preserve activity across the shelf life of the components.