Human MyD88 PharmaGenie ELISA Kit (SBRS0906)

The Assay Genie MyD88 PharmaGenie ELISA is a quantitative immunoassay for human Myeloid Differentiation Primary Response 88 (MyD88), a 33 kDa cytosolic adapter protein that couples Toll-like receptor (TLR) and IL-1 receptor family signaling to downstream IRAK kinases, driving NF-κB and MAPK pathway activation. The kit supports research into innate immunity, septic shock, autoimmune inflammation, and TLR ligand responses in cell culture supernatants, plasma, and serum samples.

Each kit supplies a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP reagents, and calibrated standards. The protocol uses a 100 uL sample volume per well and produces a colorimetric signal readable on a standard plate reader; reagents are stored refrigerated with standards and conjugate aliquoted to limit freeze-thaw exposure. For research use only, the assay leverages a human-specific capture antibody that exhibits no significant cross-reactivity with related TLR adapter homologs, providing reproducible quantification of MyD88 protein across sample matrices.