Human MutL Homolog 1 (MLH1) ELISA Kit

MLH1 (MutL Homolog 1) is a pivotal DNA mismatch repair protein whose loss of function drives Lynch syndrome (hereditary nonpolyposis colorectal cancer) and contributes to sporadic colorectal, endometrial, ovarian, and gastric tumors. The Assay Genie MLH1 ELISA Kit is a quantitative immunoassay built for research into MMR pathway integrity, microsatellite instability, and tumor suppressor biology, enabling direct measurement of MLH1 protein in tissue homogenates and cell lysates prepared from cultured cells or patient-derived specimens.

Researchers studying chemoresistance, hereditary cancer syndromes, or biomarker validation in tumor biopsies can profile mismatch repair status by running this kit alongside MSH2, MSH6, and PMS2 readouts. Detection is read on a standard microplate reader using a colorimetric endpoint, and the complete kit supplies a pre-coated plate, lyophilized standards, detection antibody, HRP conjugate, wash buffer, substrate, and stop solution in sealed vials sufficient for 96 assays per box.

Kit components should be refrigerated when not in use and protected from light where indicated; reconstituted standards are best aliquoted to limit repeated freeze-thaw cycles. The assay is intended for research use only and is not cleared for diagnostic or clinical decision-making.