Human Mucin 5 Subtype B (MUC5B) ELISA Kit

This quantitative immunoassay from Assay Genie measures human MUC5B levels in tissue homogenates, cell lysates, and cell culture supernatants. MUC5B is a large, gel-forming secreted mucin produced by submucosal glands of the conducting airways and salivary tissue; altered MUC5B expression is implicated in idiopathic pulmonary fibrosis, chronic obstructive pulmonary disease, muco-obstructive lung disorders, and certain upper gastrointestinal pathologies.

The microplate-based workflow produces a colorimetric readout compatible with standard laboratory plate readers. Researchers studying airway biology, mucosal immunology, and respiratory disease apply this kit to quantify secreted mucin in bronchoalveolar lavage samples, sputum, and cell-based models of goblet-cell hyperplasia.

Supplied reagents include a pre-coated microplate, detection antibody, standard dilution buffer, wash concentrate, chromogenic substrate, and stop solution. Bound analyte is detected through a stepwise incubation, and absorbance values are read after substrate conversion. For research use only.