Human MTRNR2L6/Humanin-like protein 6 ELISA Kit

A competitive ELISA kit from Assay Genie designed for the accurate quantitative detection of Humanin-like protein 6 (MTRNR2L6) in human samples. The assay is coated with antigen and offers a sensitivity of 0.188 ng/mL with a detection range of 0.313–20 ng/mL. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid samples.

The kit supports research into Humanin-like protein 6, a protein implicated in cellular functions such as cell survival and mitochondrial protection, with dysregulation linked to metabolic disorders, neurodegenerative diseases, and aging. It is reactive with human samples and should be stored sealed at 2–8°C, without cryopreservation. Linearity has been demonstrated for serum, EDTA plasma, and heparin plasma across multiple dilutions, with corresponding recovery data also reported.

The competitive ELISA workflow involves equilibrating the TMB substrate, adding standards or samples with biotin-labelled antibody, incubating at 37°C, washing, developing colour with TMB in the dark, stopping the reaction, and reading absorbance at 450 nm. Sample preparation guidance is provided for serum, plasma, cell culture supernatant, cell lysates, and tissue homogenates. Supplied components include an ELISA microplate, lyophilised standard, biotin-labelled antibody, HRP-streptavidin conjugate, TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, stop solution, wash buffer, plate sealer, and a technical manual.