Human MTHFS ELISA Kit (HUFI07565)

The methenyltetrahydrofolate synthetase (MTHFS) protein-quantification assay from Assay Genie supports research into one-carbon folate metabolism, where MTHFS catalyzes the ATP-dependent conversion of 5-formyltetrahydrofolate to 5,10-methenyltetrahydrofolate, a step that channels reduced folate derivatives back into the tetrahydrofolate pool used for purine and thymidylate biosynthesis.

Investigators studying antifolate chemotherapy response, neural tube defect risk, and cellular folate trafficking use this plate-format kit to track changes in MTHFS abundance in human cell lines, tissue homogenates, and primary culture lysates. Each kit contains a pre-coated microplate, lyophilized reference standards in sealed vials, biotinylated detection antibody, streptavidin-HRP conjugate, concentrated wash buffer, TMB substrate solution, and stop reagent, all intended for research use only in laboratory settings.

Reconstituted standards and diluted samples are pipetted into the wells as single-use aliquots; bound MTHFS is revealed through the sequential antibody binding reaction, and absorbance is recorded on a standard plate reader for interpolation against the standard curve. Unopened kit components should be stored refrigerated according to the package insert, and reconstituted reagents are kept cold and protected from light between runs to preserve activity.