Human MTHFD1 ELISA Kit (HUFI07562)

Quantification of the cytoplasmic trifunctional methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) protein, encoded on chromosome 14q24, underpins research into cellular one-carbon flux. The Assay Genie kit provides a complete ELISA workflow built around a pre-coated 96 Assays microplate, paired monoclonal capture and detection antibodies, a lyophilized standard curve set, TMB-based chromogenic substrate, stop solution, and concentrated wash buffer ready for immediate reconstitution. The trifunctional MTHFD1 polypeptide harbors dehydrogenase, cyclohydrolase, and synthetase domains that sequentially convert formate and tetrahydrofolate to 10-formyl-THF, 5,10-methenyl-THF, and 5,10-methylene-THF, feeding both nucleotide biosynthesis and S-adenosylmethionine-dependent methylation. The kit supports pharmacogenomic studies of the common R653Q (1958G>A) polymorphism linked to neural tube defect risk and altered chemosensitivity, antifolate research using methotrexate and 5-fluorouracil, and oncology programs profiling nucleotide-demand tumors. Using double-antibody immunoassay methodology with the included calibrators, researchers can interpolate endogenous MTHFD1 concentrations from blood-derived specimens, and the absorbance readout is compared against a seven-point standard curve for quantitative analysis. Unopened kit components are stored at 4 °C; the standard vial and detection antibody concentrate should be kept at -20 °C for extended shelf life. The format suits cohort screening, case-control stratification, and longitudinal treatment monitoring designs where MTHFD1 abundance shifts with pharmacological intervention or folate dietary manipulation.