Human MTBP/Mdm2-Binding Protein  ELISA Kit

Designed for the quantitative measurement of human MTBP (Mdm2-binding protein), this Sandwich ELISA kit from Assay Genie is configured for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay uses a double-antibody sandwich format with colorimetric detection, and is intended for research applications including studies of the MDM2–p53 pathway, where MTBP plays a role in regulating MDM2 stability and p53/TP53 homeostasis. Synonyms listed for the target include MTBP, Mdm2-binding protein, hMTBP, and MDM2BP, and the associated UniProt entry is Q96DY7.

Performance characteristics quoted by the manufacturer include a sensitivity of 0.188 ng/mL and a working range of 0.313–20 ng/mL. Linearity in serum (n=5) is reported at 85–103% (1:2), 84–99% (1:4) and 81–100% (1:8); in EDTA plasma (n=5) at 95–105% (1:2), 87–94% (1:4) and 86–98% (1:8); and in heparin plasma (n=5) at 93–102% (1:2), 84–94% (1:4) and 85–99% (1:8). Spike-and-recovery values are 90–102% (average 94%) for serum, 86–105% (average 94%) for EDTA plasma, and 90–99% (average 93%) for heparin plasma. The kit is stored at 2–8°C for 12 months, and the research area is given as Cell Cycle.