Human  Msln (Mesothelin) ELISA Kit (HUFI03386)

The capture-and-detection immunoassay workflow completes in approximately 3.5 hours from loading to final absorbance read, with a 100 uL sample volume added to each pre-coated well. Researchers commonly apply this kit to monitor circulating MSLN levels in patients enrolled in mesothelioma clinical cohorts, to track treatment response in ovarian and pancreatic cancer trials, and to validate mesothelin-targeted CAR-T and antibody-drug conjugate candidates in translational studies. Assay Genie recommends running all standards and unknowns in duplicate, and intra-assay precision falls below 10% CV with recovery of spiked recombinant mesothelin in the 85-115% range across tested sample matrices.