Human MSLN (Mesothelin) ELISA Kit (HUES02557)

This sandwich ELISA delivers quantitative readouts with an analytical sensitivity typically in the low pg/mL range, a dynamic range spanning roughly 0.16 to 10 ng/mL of recombinant MSLN, and intra-assay and inter-assay coefficients of variation generally below 10%. A standard run uses 100 µL of diluted serum, plasma, cell culture supernatant, or other biological fluid per well, with the full protocol completed in approximately 3.5 to 4.5 hours at room temperature.

Researchers commonly deploy this Assay Genie kit in oncology biomarker studies that measure soluble mesothelin in serum from mesothelioma, pancreatic, ovarian, and lung adenocarcinoma cohorts, and in cell-based experiments where shed MSLN is quantitated in conditioned media after cytokine, drug, or siRNA treatment. The kit is also used to confirm MSLN knockdown or overexpression in transfected cell lines, to compare MSLN release across tumor cell lines, and to support pre-clinical development of antibody-drug conjugates, CAR-T cells, and therapeutic vaccines that target MSLN-positive tumors.

For best results, equilibrate all reagents to room temperature before use, avoid bubbles when pipetting, and run samples in duplicate with an eight-point standard curve on each plate. The streptavidin-HRP conjugate and biotinylated detection antibody are supplied in volumes sufficient for the full 96-well plate, and reconstituted standards should be aliquoted and stored frozen to limit freeze-thaw cycles.