Human MRP1 ELISA Kit

From Assay Genie, this Sandwich ELISA is designed for the quantitative measurement of human MRP1 (Multidrug Resistance Protein 1), a key protein involved in multidrug resistance that mediates the efflux of various chemotherapeutic agents from cells. The kit is intended for use with human samples including serum, plasma, cell culture supernatant, and cell or tissue lysate.

Assay performance characteristics include a sensitivity of 0.188 ng/mL and a standard range of 0.313-20 ng/mL, with absorbance read at 450 nm following TMB substrate development. The workflow involves a primary incubation at 37°C, biotinylated detection antibody binding, HRP-Streptavidin binding, and TMB color development. Kit components include a pre-coated microplate, lyophilized standard, biotin-labeled antibody (100X), HRP-Streptavidin conjugate (SABC, 100X), TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, stop solution, 25X wash buffer, and plate sealer.