Human MRFAP1 ELISA Kit (HUFI07573)

Chromatin biology research frequently requires accurate measurement of proteins that regulate histone acetylation. This dedicated assay from Assay Genie enables quantitative detection of MRFAP1 (Mof4 family associated protein 1), a nuclear factor that associates with the MOF/KAT8 histone acetyltransferase complex. MRFAP1 modulates MOF activity and has been linked to chromatin remodeling, DNA damage response, and transcriptional regulation, making its measurement valuable in studies of epigenetic mechanisms.

The kit is configured for use with biological fluid specimens in a microplate-based workflow with a colorimetric endpoint. Pre-coated plate wells, calibration standards, detection reagents, and substrate are supplied in sufficient quantity for 96 individual sample determinations under standard assay conditions. A standard curve generated from the calibrators enables interpolation of unknown sample concentrations.

Reagents should be stored refrigerated to preserve activity through the kit's shelf life. The protocol involves sequential incubation and wash steps compatible with typical laboratory equipment, and results can be read on a standard microplate reader. By providing a targeted detection tool for an under-characterized chromatin regulator, the kit supports investigations into histone modification pathways, gene expression control, and the molecular basis of diseases involving epigenetic dysregulation.