Human MPPED1 ELISA Kit (HUFI07589)

The MPPED1 protein, encoded by the metallophosphoesterase domain containing 1 gene, is a cytoplasmic metallophosphoesterase linked to neural differentiation pathways and reported as dysregulated in several solid tumor investigations. This Assay Genie kit provides an absorbance-based immunoassay for quantifying human MPPED1 in laboratory research samples, supporting investigators working on developmental biology, oncology biomarker discovery, and gene expression surveys at the protein level.

Configured as a 96 Assays kit with pre-coated microplate wells, calibrated standards, detection antibody, and substrate solution, the workflow uses a standard microplate reader. Researchers add diluted samples and standards to wells, introduce a biotinylated detection reagent, wash, incubate with streptavidin-HRP conjugate, add stop solution, and read absorbance to interpolate MPPED1 concentration against a standard curve.

Reagents should be refrigerated on arrival and equilibrated to room temperature before each run; avoid repeated freeze-thaw cycles of diluted working solutions and protect light-sensitive components from exposure. Intended for research use only, the assay supports analysis of MPPED1 in cell lysates, tissue homogenates, and conditioned media from human cell lines, including work on cancer cell models, neural progenitor populations, and patient-derived specimens relevant to solid tumor and neurodevelopmental studies.