Human MP-IgM (Mycoplasma pneumoniae-Immunoglobulin M) ELISA Kit (HUFI04715)

Built on a μ-capture (trapping) immunoassay format, this Assay Genie kit uses anti-human IgM antibodies pre-adsorbed to the microplate to selectively capture circulating IgM, minimising interference from rheumatoid factor and competing IgG that often compromise direct-coating IgM assays. The kit supports up to 96 determinations and is supplied for in vitro research use only.

Mycoplasma pneumoniae is a leading cause of community-acquired atypical ("walking") pneumonia, and IgM serology is widely used in clinical research to identify acute or recent infection, as IgM titres typically rise within the first one to two weeks after symptom onset. The trapping design is particularly useful for early-window samples from paediatric and adolescent patient cohorts, where timely laboratory confirmation supports studies of outbreak dynamics, antibiotic stewardship, and respiratory pathogen surveillance.

Reagent volumes and incubation steps follow a standard indirect immunoassay workflow with a colour-developing substrate read on a standard microplate reader; full procedural detail, run-specific calibration guidance, and interpretation notes are provided in the product insert included with the kit.