Human/Mouse/Rat Phospho-Stat 3 (Y705) PharmaGenie ELISA Kit (SBRS1987)

The Phospho-Stat 3 (Y705) PharmaGenie ELISA Kit from Assay Genie is a phosphorylation-specific immunoassay that selectively detects STAT3 when phosphorylated at the activating tyrosine 705 residue, a key node in the JAK/STAT signaling cascade triggered by cytokines such as IL-6, IL-10, and EGF. Researchers apply this ELISA to quantify activation of STAT3 in cell lysates and tissue lysates from human, mouse, and rat samples, supporting studies in oncology, immunology, and inflammation research. The colorimetric assay is supplied in a microplate format with pre-coated capture wells, delivering quantitative measurement of phospho-STAT3 that can be normalized to total STAT3 when run alongside a companion whole-protein ELISA. Unopened kit components are shipped refrigerated; reconstituted detection antibody and standards should be aliquoted into single-use vials and stored at -20 degrees C to limit repeated freeze-thaw cycles, while the TMB substrate must be protected from light. Each kit includes a standardized phosphoprotein calibrator, biotinylated detection reagent, streptavidin-HRP conjugate, wash buffer, and stop solution, and is validated with lysates prepared in non-denaturing buffer supplemented with phosphatase and protease inhibitors to preserve the labile Y705 phospho-epitope. Typical sample loading is approximately 100 uL of clarified lysate per well, with an assay runtime of roughly 3 to 4 hours at room temperature.