Human/Mouse/Rat Phospho-Stat 3 (Y705) and Total Stat 3 PharmaGenie ELISA Kit (SBRS1988)

By quantifying phospho-Stat 3 (Y705) and total Stat 3 within the same wells, this Assay Genie kit allows researchers to calculate phosphorylation stoichiometry and normalize pathway activation to total protein, eliminating inter-well variability that affects parallel single-target phospho assays. The Y705 residue is the canonical JAK-dependent phosphorylation site triggered by IL-6, IL-10, and growth factor stimulation.

Validated for cell lysate and tissue lysate workflows with human, mouse, and rat specimens, the kit supports oncology, inflammation, and immune regulation research where STAT3 hyperactivation is implicated in tumor progression and cytokine signaling dysregulation. The colorimetric readout is compatible with standard microplate readers.

Reagents are supplied for 96 assays in a microplate format. Store kit components at -20°C, protect from light, and aliquot lysate samples before use to limit freeze-thaw cycles. Research use only.