Human/Mouse/Rat Phospho-SMAD2 (SER245/250/255) PharmaGenie ELISA Kit (SBRS1970)

The Phospho-SMAD2 (Ser245/250/255) PharmaGenie ELISA Kit from Assay Genie quantifies activated SMAD2 protein in cell lysates and tissue lysates. SMAD2 phosphorylation at the C-terminal Ser245, Ser250, and Ser255 residues is catalyzed by the type I TGF-beta receptor (ALK5) upon ligand binding, serving as a direct biochemical indicator of canonical TGF-beta/SMAD signal transduction.

Researchers working on fibrosis, epithelial-mesenchymal transition, cancer invasion, and cytokine-mediated inflammation track this phospho-specific readout to monitor pathway activation in cultured cells and tissue specimens. The microplate-based format supports multiple experimental replicates, with 37°C incubation during the primary antibody binding step. Absorbance values are normalized to total protein concentration for quantitative comparison between treatment groups and vehicle controls.

Reagents ship refrigerated and should be stored according to the included manual to preserve capture antibody activity across repeated freeze-thaw cycles. Cross-reactivity has been validated for human, mouse, and rat samples, enabling comparative work in primary cultures, established cell lines, and tissue biopsies from translational disease models.