Human/Mouse/Rat Phospho-SMAD2 (SER245/250/255) and Total SMAD2 (SBRS1971)

From Assay Genie, this kit enables simultaneous measurement of phosphorylated SMAD2 at serine residues 245, 250, and 255 alongside total SMAD2 protein levels in a single experiment. SMAD2 serves as a critical intracellular transducer of TGF-beta signaling, and its phosphorylation at the C-terminal SSXS motif reflects receptor activation and downstream pathway activity. By providing parallel phospho- and total-protein readouts, this dual-detection format supports accurate normalization without requiring separate control assays, which is valuable when working with limited sample material from cell lysates or tissue lysates.

This kit is used in research on fibrotic, inflammatory, and oncogenic signaling cascades, and it is reactive with human, mouse, and rat samples. Investigators use the ELISA to monitor TGF-beta pathway dynamics in response to ligand stimulation, receptor inhibition, or small-molecule screening. Standard ELISA workflow is followed, with absorbance read on a standard microplate reader. Store all components according to the supplied protocol; conjugate and standard require storage at -20 degrees C to preserve activity, while the microplate and buffers are kept refrigerated at 4 degrees C. Allow frozen samples and reagents to thaw completely on ice before use and avoid repeated freeze-thaw cycles to maintain epitope integrity.

Each kit contains pre-coated wells, detection antibodies specific for the Ser245/250/255 phospho-epitope and total SMAD2, standard proteins, and substrate solution. Typical run time is approximately 3-4 hours from sample addition to stop reaction. The cell and tissue lysate input requirement allows direct analysis of signaling events in cultured cells or harvested tissues without prior enrichment.