Human/Mouse/Rat Phospho-RPS6 (S235/S236) PharmaGenie ELISA Kit (SBRS1953)

Ribosomal protein S6 phosphorylated at serines 235 and 236 is a well-characterized downstream indicator of mTORC1 pathway activity. The Phospho-RPS6 (S235/S236) PharmaGenie ELISA Kit from Assay Genie provides a quantitative readout of this modification in cell lysates and tissue lysates, supporting studies of translation control, cell growth, autophagy, and oncogenic signaling across human, mouse, and rat samples.

Researchers apply the kit to track mTOR inhibitor response (rapamycin, everolimus, Torin), compare basal versus stimulated phosphorylation after growth factor or amino acid challenge, and confirm pathway engagement in preclinical drug screens. It is also used to verify CRISPR knockout phenotypes and to benchmark Western blot or phospho-flow data against a quantitative ELISA value.

Each kit provides reagents for 96 assays in a microplate format with an absorbance-based colorimetric read. Samples should be prepared in lysis buffer containing phosphatase inhibitors to preserve the phospho-epitope, and storage recommendations are supplied with the kit. The protocol is optimized for whole-cell and tissue lysate inputs, making it suitable for cancer cell lines, primary cultures, and animal tissue.