Human/Mouse/Rat Phospho-Raf-1 (Ser301) PharmaGenie ELISA Kit (SBRS1943)

The Phospho-Raf-1 (Ser301) ELISA Kit from Assay Genie quantitatively measures phosphorylated Raf-1 kinase at serine 301, a regulatory autophosphorylation site within the MAPK/ERK signaling cascade. Raf-1 activation propagates downstream through MEK and ERK phosphorylation, governing cellular responses including proliferation, survival, and differentiation. Detecting Ser301 phosphorylation supports studies of oncogenic Ras-Raf signaling, including investigations in melanoma and other tumors carrying pathway mutations. The kit accepts cell lysates and tissue lysates, enabling experiments in cultured mammalian cells and in vivo tissue models. Researchers apply the assay to monitor pathway activity following growth factor stimulation, oncogene expression, or pharmacological kinase inhibitor treatment, allowing comparison of phospho-Raf-1 levels across treated versus untreated conditions. Each kit includes the essential reagents and buffers required for the ELISA workflow.