Human/Mouse/Rat Phospho-Raf-1 (Ser301) and Total Raf-1 PharmaGenie ELISA Kit (SBRS1944)

Raf-1 kinase phosphorylation at Ser301 functions as a measurable readout of ERK-mediated feedback within the canonical RAF/MEK/ERK signaling cascade, and the Assay Genie Phospho-Raf-1 (Ser301) and Total Raf-1 PharmaGenie ELISA Kit gives laboratories a quantitative immunoassay for tracking both the phosphorylated and total forms of the kinase from a single lysate preparation. Raf-1 (also called C-Raf) sits at the MAP3K tier of the pathway, with phosphorylation at Ser301 characterized as a feedback modification that attenuates upstream Ras-Raf signaling output. Quantifying the phospho-Raf-1 (Ser301) to total Raf-1 ratio therefore lets researchers monitor pathway activation status alongside feedback regulation in the same experimental set.

The microplate-format kit is configured for use with cell lysates and tissue lysates prepared in standard lysis buffers, and supports experiments in cancer cell signaling, neurodegeneration, receptor tyrosine kinase biology, and small-molecule kinase inhibitor screening. By resolving the two analytes in parallel, the dual-detection design lets users normalize phosphorylation changes to total protein abundance rather than relying on separate loading controls. Laboratories working on BRAF- or RAS-driven signaling, MEK inhibitor pharmacology, or downstream ERK pathway analysis will find the matched phospho/total readouts useful for separating activation effects from expression-level differences.

Shipped as a 96-assay pack, the kit arrives with the microplate, capture and detection reagents, buffers, and reference standards needed for routine bench work. The protocol is written for absorbance-based reading on standard plate readers in academic and core facility settings. Because the assay measures an endogenous phosphorylation event in cell and tissue lysates, it slots cleanly into pathway stimulation time courses, kinase inhibitor washout studies, and targeted phospho-proteomic workflows where Western blotting for phospho-Raf-1 has traditionally been the standard approach.