Human/Mouse/Rat Phospho-PTEN (S380) and Total PTEN PharmaGenie ELISA Kit (SBRS1939)

The Assay Genie dual-analyte immunoassay quantifies phosphorylated PTEN at the serine 380 regulatory site alongside total PTEN protein in a single plate run, enabling normalization of phosphorylation status to total expression within the same lysate. Phosphorylation at S380 within the PTEN C-terminal tail is a key regulatory event that stabilizes the closed, less-active conformation and is implicated in PI3K/AKT pathway control and tumor suppressor biology. Lysate-based sample handling suits cultured cell and tissue homogenate workflows, and the microplate format supports parallel processing of standard curves plus experimental samples. Standard loading volumes of approximately 50 µL of clarified lysate per well allow direct comparison between phospho-PTEN and total PTEN signals on the same plate. Reagents should be refrigerated upon arrival, equilibrated to room temperature before use, and protected from light to preserve antibody activity across repeated freeze-thaw cycles. Researchers studying cancer signaling, insulin response, or phosphatase regulation will appreciate the ability to derive a phospho-to-total ratio from matched samples, reducing inter-assay variability that arises when comparing separate singleplex ELISA runs.