Human/Mouse/Rat Phospho-P70S6K (T421/S424) PharmaGenie ELISA Kit (SBRS1919)

Built by Assay Genie, this phosphorylation ELISA quantifies the activated state of p70 S6 kinase by detecting phosphorylation at threonine 421 and serine 424, two regulatory residues whose modification reflects upstream mTORC1 activity. Researchers investigating insulin signaling, growth factor responses, rapamycin pharmacology, or oncogenic pathway activation in tumor models commonly apply the kit to whole-cell and tissue lysates as a quantitative alternative to western blotting. The capture antibody configuration preferentially recognizes the dually phosphorylated kinase, allowing discrimination between basal and stimulated states in serum-starved and restimulated cell experiments. Each plate includes recombinant phosphoprotein standards for accurate interpolation across the working range, and the assay tolerates lysates prepared in RIPA-style buffers supplemented with phosphatase inhibitors to preserve site occupancy during handling. Reconstituted standards and prepared lysates should be kept refrigerated at 4 degrees C and the antibody-coated plate stored dry until use.