Human/Mouse/Rat Phospho-P70S6K (T421/S424) and Total P70S6K PharmaGenie ELISA Kit (SBRS1921)

The PharmaGenie ELISA from Assay Genie is a dual-analyte immunoassay that simultaneously quantifies phosphorylated P70S6K at threonine 421 and serine 424 alongside total P70S6K protein in the same well. This co-detection format enables researchers to directly calculate phospho-to-total ratios, which is the standard readout for mTORC1 pathway activation downstream of rapamycin-sensitive signaling cascades. The kit is validated for use with cell lysates and tissue lysates from human, mouse, and rat origins, supporting cross-species studies in cancer biology, metabolic disease, and muscle physiology research. The assay employs a colorimetric readout with sufficient sensitivity to detect endogenous kinase levels in cultured mammalian cells and snap-frozen tissue biopsies, making it appropriate for experiments involving insulin, growth factor, or amino acid stimulation of the mTOR pathway.