Human/Mouse/Rat Phospho-P53 (S15) PharmaGenie ELISA Kit (SBRS1917)

Phosphorylation of p53 at serine 15 is a hallmark of the early DNA damage response, catalyzed by ATM, ATR, and DNA-PKcs kinases after genotoxic insults such as ionizing radiation, UV exposure, and chemotherapeutic treatment. The Assay Genie Human/Mouse/Rat Phospho-P53 (S15) PharmaGenie ELISA Kit is a quantitative ELISA that measures this modification in cell lysates and tissue lysates, supporting research into genome surveillance, tumor suppressor pathway activation, apoptosis induction, and oncology drug screening workflows.

The kit is configured as a 96-assay plate format and includes a phospho-specific capture antibody, a matched detection antibody, recombinant phospho-p53 protein standard, TMB substrate, wash buffer, and stop solution for a complete run. Validated for cross-reactivity across human, mouse, and rat samples, the antibodies recognize the phospho-S15 epitope in each species, enabling translational comparison between cell-line experiments and rodent disease models. The assay is for research use only and is intended for laboratory investigation of p53 signaling rather than clinical diagnostic use.