Human/Mouse/Rat Phospho-NF-KB p65 (S536) PharmaGenie ELISA Kit (SBRS1911)

This phosphorylation ELISA from Assay Genie quantifies activation of the NF-κB transcription factor pathway by detecting phosphorylation at Serine 536 on the p65 (RelA) subunit. Ser536 phosphorylation is driven by the IKK complex and serves as a widely studied marker of canonical NF-κB signaling across inflammation, immune response, cytokine signaling, oncology, and cellular stress research.

Formatted for 96 assays, the kit is applied to cell lysates and tissue lysates, supporting signaling studies in cultured mammalian cells and animal model tissues. Researchers use this phosphorylation ELISA to monitor pathway activation kinetics, compare treatment conditions, screen compounds that modulate NF-κB activity, and validate phosphorylation events first observed by Western blot.

Indicated for use with Human, Mouse, and Rat samples as reflected in the product name, this kit supports translational comparison between species within a single workflow. By converting a phospho-specific Western blot readout into an ELISA workflow with 96 assays per kit, it offers higher sample throughput while retaining residue-level discrimination between phosphorylated and total p65.

Each kit ships with the reagents required to measure phosphorylated p65 in cell or tissue lysates. Refer to the product insert for storage temperature, handling instructions, and shelf life upon receipt. For research use only.