Human/Mouse/Rat Phospho-MKK6 (S207) PharmaGenie ELISA Kit (SBRS1898)

The Phospho-MKK6 (S207) ELISA Kit from Assay Genie quantifies the activated, serine-207-phosphorylated form of MAP kinase kinase 6, a pivotal upstream activator of the p38 MAPK signaling cascade that transmits cytokine, oxidative, and osmotic stress signals into cellular responses. Because phosphorylation at S207 marks the catalytically competent kinase, this assay reports on pathway engagement rather than bulk MKK6 abundance, making it useful for kinetic studies of p38 MAPK activation in stimulated cell systems.

Configured for human, mouse, and rat samples, the kit accepts cell lysates or tissue lysates as input and supports translational workflows linking in vitro cell line data to in vivo animal model findings. Common research applications include tracking inflammatory signaling in macrophage or microglia stimulation experiments, profiling cancer cell responses to chemotherapeutics or cytokines, and examining stress responses in cardiac, neuronal, or hepatic tissues.

Each kit provides a pre-coated antibody plate, phospho-selective detection reagents, calibrated recombinant standard, wash buffer, and substrate sufficient for the full microplate format. Store unopened components refrigerated at 4 °C; reconstituted standards should be aliquoted and kept frozen to limit freeze-thaw cycles and preserve antibody binding activity. Intended for research use only.