Human/Mouse/Rat Phospho-MKK6 (S207) PharmaGenie ELISA Kit and Total MKK6 PharmaGenie ELISA Kit (SBRS1899)

Phospho-MKK6 (S207) and Total MKK6 Dual-Readout Signaling ELISA

Mapping activation of the p38 MAP kinase stress-response pathway requires measuring both the phosphorylated and total forms of upstream kinases in the same samples. MKK6, also called MAP2K6, is the dual-specificity activating kinase for p38 MAPK, and phosphorylation at S207 reflects pathway activation triggered by cytokines, osmotic shock, and UV exposure. The Phospho-MKK6 (S207) and Total MKK6 PharmaGenie ELISA Kit from Assay Genie delivers parallel quantification of phospho-MKK6 (S207) and total MKK6 protein from cell lysates and tissue lysates, allowing researchers to normalize pathway activation to total kinase expression across treatments. The microplate format supplies capacity for 96 individual assay determinations, supporting in vitro pharmacology, signal transduction, and kinase inhibitor profiling workflows.

Applications include inflammation research, cellular stress studies, and characterization of upstream activators and downstream effectors within the MKK6-p38 signaling axis. The reagents are intended for research use only.