Human/Mouse/Rat Phospho-MKK3 (S189) and Total MKK3 PharmaGenie ELISA Kit (SBRS1897)

This dual-analyte immunoassay from Assay Genie quantifies phosphorylated MKK3 at the activating serine 189 residue alongside total MKK3 protein in the same well, enabling direct normalization of pathway activation without separate control plates. MKK3 (also called MAP2K3 or MEK3) is an upstream kinase that activates p38 MAPK in response to inflammatory cytokines, oxidative stress, and osmotic shock, so S189 phosphorylation is widely used as a readout of cellular stress responses in pharmacology and toxicology studies.

The kit accommodates cell lysates and tissue lysates from human, mouse, and rat origins and is supplied as a complete microplate-based pack with all buffers and reagents required for 96 determinations. Researchers typically load 100 uL of prepared lysate per well, run a standard curve with the included recombinant MKK3 antigen, and read absorbance on a standard plate reader after a short substrate incubation. Reconstituted reagents should be stored refrigerated at 4 degrees C and protected from light, with the antibody-coated microplate kept desiccated until use.