Human/Mouse/Rat Phospho-Met (Y1234) PharmaGenie ELISA Kit (SBRS1894)

Quantitative phosphorylation ELISA for the activated state of the MET receptor tyrosine kinase, measuring phospho-Met at tyrosine 1234 — the conserved autophosphorylation site within the kinase activation loop. The assay accepts cell lysates and tissue lysates from human, mouse, and rat samples, supporting cell signaling and oncology workflows, including HGF/MET pathway activation, downstream signaling through MAPK and PI3K/AKT cascades, and receptor turnover under kinase inhibitor or HGF ligand challenge.

From the Assay Genie PharmaGenie reagent line, each kit supplies 96 assays in a microplate format using a colorimetric readout, so phospho-Met (Y1234) levels from treated and untreated arms can be normalized to total protein or to paired total-Met readings from the same lysate. Typical applications include drug-response profiling in tumor cell models, time-course monitoring of pathway reactivation after kinase inhibitor washout, and comparative receptor phosphorylation across xenograft or syngeneic tumor tissue lysates. For research use only; not for use in diagnostic procedures.