Human/Mouse/Rat Phospho-Jak1 (Y1022)& Total JAK1 PharmaGenie ELISA Kit (SBRS1868)

Signaling researchers working on the JAK/STAT pathway can measure both phosphorylated and total JAK1 from a single sample with this dual-analyte ELISA kit from Assay Genie. The Phospho-Jak1 (Y1022) and Total JAK1 assay quantifies activation at the Y1022 loop alongside total JAK1 protein, supporting normalization without running a separate total-protein plate. This dual readout is widely adopted in cytokine signaling research, autoimmune disease models, and oncology programs evaluating JAK inhibitors.

Validated for cell lysates and tissue lysates, the kit accepts lysis buffers commonly used in western blot workflows, so existing lysate preparations can be reused. Measuring phospho and total targets from one aliquot reduces hands-on time, limits freeze-thaw cycles on precious samples, and minimizes inter-assay variability.

Each kit supplies reagents for 96 assays, accommodating both small pilot studies and larger screening campaigns. Investigators probing IL-6, interferon, or growth factor pathways benefit from the parallel measurement, enabling more accurate quantification of pathway activation after stimulation or compound treatment.