Human/Mouse/Rat Phospho-Jak1 (Y1022) PharmaGenie ELISA Kit (SBRS1867)

JAK1 (Janus kinase 1) is a cytoplasmic tyrosine kinase that mediates cytokine receptor signaling through the JAK/STAT pathway, including interferon, interleukin-6, and growth hormone responses. Phosphorylation at tyrosine 1022 within the activation loop is a direct indicator of receptor-driven kinase activation, making this PharmaGenie ELISA a useful tool for quantifying signal transduction dynamics in cell lysates and tissue lysates. The Assay Genie kit employs a phosphorylation-specific capture antibody that selectively recognizes JAK1 only when phosphorylated at Y1022, allowing researchers to monitor active kinase pools without requiring Western blot analysis. The colorimetric microplate workflow uses a pre-coated plate design, and samples are prepared in lysis buffer compatible with phosphoprotein preservation. Cross-reactivity has been validated for human, mouse, and rat JAK1, supporting comparative studies across model systems and primary cultures from different species.