Human/Mouse/Rat Phospho-HDAC4 (S632) (SBRS1850)

Assay Genie's Phospho-HDAC4 (S632) ELISA Kit targets the phosphorylated serine 632 residue on class IIa histone deacetylase 4, a regulatory mark linked to 14-3-3 binding and nucleocytoplasmic shuttling. The assay cross-reacts with mouse and rat HDAC4 orthologs, supporting translational work across species. It accepts cell lysates or tissue lysates prepared in detergent-based buffers, with a recommended sample volume of 100 uL per well. Researchers studying cardiac hypertrophy, skeletal muscle differentiation, neuronal stress responses, and glucocorticoid-driven transcriptional repression can monitor S632 site occupancy downstream of CaMK and protein kinase D signaling. The microplate and detection reagents should be stored refrigerated until use, while reconstituted standards require frozen storage at -20 degrees C. Each kit provides 96 assays in a strip-well microplate format with all required buffers and a positive control lysate included.