Human/Mouse/Rat Phospho-GSK3b (S9) and Total GSK3b PharmaGenie ELISA Kit (SBRS1845)

This dual-analyte immunoassay from Assay Genie simultaneously quantifies phosphorylated GSK3β at Serine 9 and total GSK3β protein from a single well, enabling direct calculation of phosphorylation ratios without separate normalization runs. The panel is reactive with human, mouse, and rat specimens, broadly applicable across cell-line and rodent model studies investigating insulin signaling, Wnt pathway activity, tau hyperphosphorylation in Alzheimer's models, and glycogen metabolism.

Cell lysates and tissue lysates prepared with standard RIPA buffer are compatible inputs. The protocol runs in approximately 4.5 hours using a 100 μL sample volume per well, with capture antibodies pre-coated on the plate. GSK3β migrates near 46 kDa on standard western blots, and this assay offers a quantitative ELISA alternative for laboratories tracking kinase regulation. Researchers in oncology, neurobiology, and metabolic disease frequently deploy this panel to monitor downstream responses to kinase inhibitors, growth factor stimulation, and siRNA-mediated knockdown of upstream regulators such as Akt and PI3K.