Human/Mouse/Rat Phospho-GSK3a (S21) PharmaGenie ELISA Kit (SBRS1842)

This phosphorylation-specific ELISA from Assay Genie quantitatively detects GSK3α (glycogen synthase kinase 3 alpha) when phosphorylated at serine 21, the AKT-mediated inhibitory site that sits at a key regulatory node in insulin, PI3K/AKT, and Wnt/β-catenin signaling cascades. The kit is validated across human, mouse, and rat samples, supporting cross-species comparative studies in metabolic disease, neuroscience, and oncology research.

Phosphorylation at S21 suppresses GSK3α catalytic activity, and quantifying the phospho-to-total ratio is a standard readout in studies of insulin resistance, Alzheimer's disease pathology, and tumor cell proliferation. Each kit provides pre-coated microplate wells, a phospho-specific capture antibody pair, and reagents sufficient for 96 determinations performed on cell lysates or tissue lysates. To preserve the labile phosphate group during sample preparation, lysates should be prepared in the presence of phosphatase inhibitors and kept on ice, with aliquots snap-frozen for storage at -80 °C and avoidance of repeated freeze-thaw cycles prior to the assay.